We have gotten our 7th Methylation Panel. He is in the genetics sheet and the methylation panel data sheet as ‘Anonymous #2.’
From his numbers he is by far the most depleted of all of us. What strange is, for a short term user, he’s genetics are not that severe (based on what Yasko says is severe). However, Anonymous #2 described himself as going through an extreme stressful period when he crashed and cited multiple sources of oxidative stress per the below quote.
I’ve also had bad experiences with NAC.
Methyl-B12, hydroxy-B12, and adenosyl-B12, however, has consistently worked to improve brainfog, dizziness and digestion. It doesn’t seem to matter which form of the three B12s I take – they all work great.
These days I’m trying to find something that can compound the improvement I’m getting from B12. Planning to increase my dose of 5-MTHF to 10mg a day, and adding in 5-formyltetrahydrofolate.
From what i can tell this regimen needs to give it some time. I tried raising Metafolin intake throughout the day but did not make a difference. Once a day P5P 50mg, TMG 1000mg, Metafolin 800mcg does the trick for me. CoQ10 100 mg per day help for Glutamate Over-excitation which could be a key problem for some of us.
So , last week I was sleeping much better and was in a good mood.
On Friday I felt on my left leg something that I could describe has a discomfort in the leg. But I have also been exercising a lot. In any case I decided to stop all the vitamins until the leg got better, because it could be for example too much B6.
The leg is now better and I’ve started reintroducing the vitamins.
What I would like to say is that Saturday I noticed I was slightly irritated. I’ve drank a few drinks on Saturday night so it’s normal I didnt sleep well. BUt then from sunday to monday and monday to tuesday (today), my mood was shit and my sleep wasn’t as good. I wasn’t so sleepy at night, even after the trazodone, and I actually tonight woke up after 5.30 hours of sleep.
I’ve restarted b12 , b9, Vitamin C and Zinc.
I think what I had was from exercise, because later on the discomfort in the leg turned into tiredness in the leg and later some small pain, which has now subsided for the most part.
Nonetheless, I would like to ask you if you had any problems with B6, or if you think 50mg a day of P5P could be problematic?
Thanks
Hi guys, where should I add my 23andme data?
Also, i’m working on a questionnaire, could you please see my thread?
viewtopic.php?f=1&t=8939
Interesting research that says low Glutathione in the brain is correlated with anhedonia in major depressive disorder.
“In vivo 1H MRS study of potential associations between glutathione, oxidative stress and anhedonia in major depressive disorder”
I just read through a very interesting paper:
“Regulation of the pentose phosphate pathway by an androgen receptor–mTOR-mediated mechanism and its role in prostate cancer cell growth”
nature.com/oncsis/journal/v3 … 1418a.html
It says that androgens, via androgen receptor, drive the Pentose Phosphat shunt, which generates NADPH, a cofactor for glutathione recycling.
Essentially, if androgen capacity is reducing (by lowered T or DHT levels or impaired Androgen Receptor by other means) you will generate less (reduced) glutathione and it will pool in the oxidized form.
This thread has cited papers that show how androgen deprivation (specifically Fin treatment) leads to oxidative stress. And how oxidative environments feedback back in different ways to effect androgen capacity.
This is paper clearly shows how reducing androgens prevents glutathione regeneration. Consistent which the data in this thread and what we expect the coming research to show.
This picture this is painting is of a vicious cycle between androgens and oxidative environment.
This is what i was saying in the P450 thread. See viewtopic.php?f=27&t=8592&start=100#p82455
D-Ribose AND ENADA NADH on the regimen is added for exactly the same reason.
Mariovitali, that is not what you were saying. It has been known for a long time that NADH/NADPH are cofactors for many reactions, and in an oxidative environment, these cofactors will shift toward their oxidized forms: NAD and NADP, removing a critical co-factor from the cellular pool.
What is novel about the above finding is that it shows (for the first time I’ve seen), that androgens drive NADPH generation.
Note that supplementing NADH will not rescue lack of NADPH. NADPH is the preferred cofactor for many important reactions. Like 5-alpha-reductase activity.
It may not be necessarily so :
So in your understanding Droit, could it be beneficial to supplement with D-Ribose and/or NADH or not?
Ribose is fun to use as sweetener. It probably will give you a little bit of energy and make up for the raw materials potentially lost to reduced Pentose Phosphate Shunt activity.
My opinion is that the 5/10mg dose of NADH is like putting out a fire with drops of water. The massive redox shift the labs in this thread are showing like mean all of that NADH is oxidizing quickly.
This thread continues to like using the pure Simplified Methylation Protocol for stabilizing the methylation cycle and shifting glutathione levels.
I see your point. I would really like to know how people are doing here by doing the methylation protocol. Do you have any feedback by any chance?
I’ve been on it for about 8 months. It has definitely helped me. I have more energy, more clear headed. I was able to completely come off of hydrocortisone as my adrenals feel much stronger. Sexual symptoms have improved maybe 10%. Not enough that I am happy, but enough that I can tell a small difference.
Overall, it is not a cure (by itself), but I feel it is definitely a large piece of the recovery puzzle.
allo people on this thread are MTHFR positive?
In Chris Kresser’s latest podcast episode he talks briefly about five key areas that have to be addressed to fix hormone imbalances - one of which is the detox system.
He mentions a mechanism I had not heard of before, in which a bad detox system (poor methylation, low glutathione etc) can result in partially metabolized hormones getting recirculated back into the system where they attach to and block receptors, but don’t have the normal effect. If this happens you can have normal hormone lab results but still have all the symptoms of low hormone levels.
This could explain why many of us have normal or low-normal testosterone lab results but exhibit the symptoms of low testosterone.
So another reason to focus on improving methylation, glutathione levels and the detox system generally.
Listen to the episode here:
chriskresser.com/the-right-and-w … -imbalance
Thank you for this post Vanish.
what is the proof for this theory. how would he knew this if lab can detect them?
Haven’t updated in a while. Unfortunately, I think I reached a certain point and slipped back.
Things were pretty good, but, the last 4 months my energy/motivation seem to decrease again. Erections weren’t as good. Night time erections seem to disappear. Tinnitus getting more annoying. I tried upping a little more B12, folate for a while and it didn’t help.
The tinnitus and a feeling in my head like it was slightly shaking, made me think it was from too much b12, folate. I stopped taking B vitamins the last few months. There has been no change to my tinnitus, slight pulsating in my head. I think this B12, folate, methylation can help to a certain point… If there was a way it can help me further, I have not found that combination.
Lately, I’ve been taking about 20mg in total of cialis/week. I found that taking vitamin C with it has a few reactions… It lessens the headache, soreness from Cialis… Slightly reduces the erection hardness… But, strangely helps with my delayed ejaculation.
Anyways, just wanted to report in. I don’t want anyone thinking I’m almost 100%.
Methylation protocol is not the solution bluejaysfan. Your “tinnitus” is NMDA Overexcitation which has to be controlled, the problem is two-fold : Methyaltion AND Low Nitric Oxide production (my theory).
Try the NO/NOx regimen and of course keep supporting methylation. All you have to do is eat around 200 gr of Spinach or Rocket every day, throughout the day (instead of eating all 200 gr at once) …I know it sounds crazy but try it, you have nothing to loose. Please do this for 10 days and report back.
Mario, I’m booked for Boston soon. Can’t wait.
I’m giving myself a break from trying anything.