Methylation Cycle and The P450 Systems

General Discussion
#44

Based on some measurements from a study, that were posted here, I made some statistics a while ago on the ratios for the Cerebral Spinal Fluid values shown:


PFS Group (# represents a subject)
#	PREG:DHEA	PREG:PROG	DHEA:PROG
1	  4,833           3,867	   0,800
2	  2,895           3,056	   1,056
3	  1,769           2,091	   1,182
Avg     3,166           3,004	   1,012
	
Control groups (# represents a subject)
#     PREG:DHEA	PREG:PROG	DHEA:PROG			
1	  6,167	  4,933	0,800
2	  5,278	  5,938	1,125
3	  6,500	  9,100	1,400
4	  4,438	  3,737	0,842
5	  4,375	  3,889	0,889
Avg     5,351	  5,519	1,011

I know there is big discrepancy between the samples, and it’s a small sample…but isn’t it too much coincidence that this seems to indicate that:

  • The ratio in the CSF between DHEA:PROG is the same in PFS and control, in average, and is almost 1:1.
  • The ratio of conversion from PREG to DHEA is impaired by the same amount as PREG to PROG in PFS sample.

This could mean there is a lack of pregnenolone

Could anyone care to comment on this…

#45

@xptriado : This is a very small sample but it’s a good start.

Update

My erections are still to 100%. Some neurological sides but the intensity lessens as time passes by. I take B12, P5P, Metafolin, TMG, Zinc, Selenium, Magnesium, Lions mane. HCG 500 iu 2xweek and my thyroid meds

I noticed that my hair is thinning and possibly i will need Minoxidil. NAC actually induced neurological side effects so Droit was right on this one. I will add AdenosylB12 (check Freddd’s thread on Phoenix Rising) and possibly Pregnenolone cream to see how things are going.

It seems that my Androgens are being used correctly (HCG-> induces P450scc-> Pregnenolone) so all is good for the time being.

I will keep you Guys posted as always.

#46

can you FEEL dht in your system, at all?

#47

Yes i believe i can. If you search my posts you will see that i discuss how my skin is more oily (especially my forehead and nose). There was a clear relationship between how oily i feel my skin and my erections.

#48

How do you take your HGC? Injections or drops? If injections is it complicated?

#49

I do injections, first couple of times were a bit difficult but now i can do a shot very easily and quickly.

#50

Mario two questions:

  • What do you use the lion’s mane for? anti oxidant properties?
  • What are you using the HCG for? To mimic LH? Doesn’t it increase your estrogen too much and give you gyno?
#51

Lion’s Mane protects from Reactive Oxygen Species, boosts NGF and attenuates Glutamate excitotoxicity.

I use HCG to boost P450scc, enhance Pregnenolone and Testosterone Production. I never saw increased E2 with HCG and this happens i believe because of the rather small dose (500 iu) that i inject. But you are right, E2 has to be monitored.

#52

CORRECTION : Lions Mane does not protect from Glutamate excitotoxicity

#53

so this lions mane could be useful as a part of the methylation regimen? because if you say it it’s like an antioxidant and same time it’s boosting NGF which probably associated with better Myelin basic protein, which comes with the improved methylation cycle, could help cure the nerves? I’m just guessing

also, did you reach any conclusion as to why forskolin stopped working? because that would be easier way to support p450scc than hcg (more easily available)

#54

Yes, this is the reason for taking Lion’s Mane. Regarding Forskolin it is quite possible that as with other medications, my body got used to it and over time it lost its efficacy. I guess that if you cycle it (say stop taking it for 5-10 days) you will not have this problem.

#55

and did it take you long to see results with the methylation protocol vitamins?

Has anyone of you, resident outside USA, ordered a 23andme ? I would like to order but I’m afraid that the distance will damage the saliva sample and so on. I live in europe

#56

It took around 10 days to see results. I am one of the people that had a 23andme test and i also live in Europe. DHL makes sure that they receive the sample on time, plus the tube has a specific agent that keeps the saliva sample in the best condition as possible until 23andme receives the specimen.

#57

How do you take your HGC? Injections or drops? If injections is it complicated?
[/quote]
I do injections, first couple of times were a bit difficult but now i can do a shot very easily and quickly.
[/quote]
With the HGC do you cycle it or follow any special protocol? Can you recommend a supplier?

#58

I usually take it for a month and then test for E2, Free T. I then stop it for around 10-15 days and restart. I never had problem of high E2 and this i believe happens because of the rather small dose of 500 IU twice weekly.

The HCG i use is called “Pregnyl” and the manufacturer is a Dutch company called N.V Organon.

#59

UPDATE

Good news Guys…i believe i am on my way to full recovery. For the first time i get consistent results for over two weeks which is a very very good sign.

Neurological side effects are slowly diminishing and their intensity wears off as time passes by. Quality of erections are stable and to 100%. No sleep problems, orgasm intensity restored some constipation still there. Incidents of irregular heartbeat are diminishing. Brain Fog gone.

Regimen :

Morning : T3/T4 for my thyroid, Metafolin 800mcg, TMG 1000 mg, P5P, Magnesium
12-ish : Zinc, Selenium, D3, K2, Iron, Lions mane (800 mg / tablet)
Lunchtime : Lions Mane
8-ish : Lions Mane
Night : Magnesium, Lions Mane

HCG : Twice a week 500 IU

1 Like
#60

Do you think we should add lion’s mane to our regimen? I mean, you’re taking quite a lot of it, so I guess it makes a difference for you. By the way, what brand do you use?

#61

can you feel it in the prostate area?

#62

It helps with boosting NGF which could be helping with faster recovery. To be honest i already ordered 2 bottles and so i am using it anyway as i didnt find any negative side effects associated with it. I will have to make a correction : each tablet is 600 mg therefore the total intake is 2400mg per day.

The brand i use is called Fungiology , i bought it from iherb.

#63

Maybe i do, but i am not sure. Some times i can feel some slight pain in the groin area but i am not sure if that is because of DHT. Since i am 44 and doing HCG i will have to re-test for PSA in the next couple of months though.