The real reason sustain alpha may work

[golf]

This points to the demethylation of the estrogen receptor, so perhaps it also has the capability to demethylate the androgen receptor as well.

Background: Aberrant DNA methylation patterns, which display global hypomethylation and local promoter hypermethylation in tumor suppressor and growth regulatory genes, are a feature of most cancers, including breast malignancy. Epigenetic fluctuations in the degree of CpG islands represent an important mechanism by which gene function is selectively activated or silenced, as a result of modifying the binding of transcription factors and methyl-DNA binding proteins. A wide range of dietary compounds, such as resveratrol, can lead to global alterations in DNA methylation. Since dietary compounds are likely to induce epigenetic rather than mutational events, assessing changes in methylation, represent excellent targets to elucidate how diet factors may modify cancer risk and tumor behavior. Resveratrol, a compound commonly found in red wine and in the Japanese knotweed plant, possesses recognized health benefits for a wide range of human diseases including cancer. Resveratrol acts as an estrogen receptor (ER) antagonist in the presence of estrogen, leading to growth inhibition of ER positive breast cancer in a dose-dependant fashion. In the absence of estradiol, resveratrol has both agonist and antagonist effects depending on the dose used. Our goal was to determine the dose-dependant methylation effects of resveratrol on ER+ breast cancer cells. Methods: MCF-7 cells were treated with 5µM, 50 µM and 100 µM of resveratrol for 36 hours, DNA and RNA were extracted and differential methylation hybridization assays were conducted to screen an array containing 8716 CpG island tags for changes in methylation. Real-time PCR was also performed to assess expression levels of DNMT1 and DNMT3b, two of the three major isoforms of identified DNA methyltransferases, based on resveratrol treatment. Results and Conclusion: 65 loci have a two-fold or greater change in methylation status. Nine of the loci are genes with known identities, one of which is the tumor suppressor gene DLC-1 which is hypermethylated in various cancers. DLC-1 was demethylated following resveratrol treatment. DNMT1 and DNMT3b levels were inhibited by resveratrol in a dose dependant fashion. Resveratrol induces methylation changes in a variety of CpG islands, including those known to be altered in human cancer. This suggests that alterations in methylation may be an important mechanism by which resveratrol provides protective effects in human disease, including ER+ breast cancer.

aacrmeetingabstracts.org/cgi/con … 05/1/647-c

Also, several papers show that resveratrol represses the androgen receptor. Perhaps via repression the AR becomes sensitive again, if sustain were removed SLOWLY the AR would then become responsive.

BACKGROUND: The chemopreventive effects of resveratrol (RSV) on prostate cancer have been well established; the androgen receptor (AR) plays pivotal roles in prostatic tumorigenesis. However, the exact underlying molecular mechanisms about the effects of RSV on AR have not been fully elucidated. A model system is needed to determine whether and how RSV represses AR transcriptional activity. METHODOLOGY: The AR cDNA was first cloned into the retroviral vector pOZ-N and then integrated into the genome of AR-negative HeLa cells to generate the AR(+) cells. The constitutively expressed AR was characterized by monitoring hormone-stimulated nuclear translocation, DNA binding, and transcriptional activation, with the AR(-) cells serving as controls. AR(+) cells were treated with RSV, and both AR protein levels and AR transcriptional activity were measured simultaneously. Chromatin immunoprecipitation (ChIP) assays were used to detect the effects of RSV on the recruitment of AR to its cognate element (ARE). RESULTS: AR in the AR (+) stable cell line functions in a manner similar to that of endogenously expressed AR. Using this model system we clearly demonstrated that RSV represses AR transcriptional activity independently of any effects on AR protein levels. However, neither the hormone-mediated nucleus translocation nor the AR/ARE interaction was affected by RSV treatment. CONCLUSION: We demonstrated unambiguously that RSV regulates AR target gene expression, at least in part, by repressing AR transcriptional activity. Repressive effects of RSV on AR activity result from mechanisms other than the affects of AR nuclear translocation or DNA binding.

ncbi.nlm.nih.gov/pubmed/19816598

[tim1911]

try it golf and let us know how you get on

[anonnn1]

It didnt work for me bud…Used it for a few months too…along with the Toco 8 and endoamp

[cdnuts]

Sustain is a staple for me, and the longer I use it, the better I feel. I’ve also been dosing with TCF-1 every twelve days on, then twelve off. It’s like night and day.

[monty]

can you update on the sustain alpha pleace cd nuts.

I am thinking of cycling it with tongkat ali 200 strength continuously if they both work!

[sanfran55]

Is anyone else feeling good on sustain alpha?

[Former]

Also, possibly worthwhile to note about Sustain Alpha is that Primordial Performance claims that the product works through “GABAergic modulation and estrogen blockage.”

primordialperformance.com/store/sustain_alpha-libido-booster.html#prodfacts

The product appears to be a mass dose of resveratrol (the touted anti-aging component). Might give this one a whirl.

[monty]

mine’s in the post!

ps ornathine, arganine, lysine combo definately helps erection strength. I can get solid wood despite having bugger all testosterone or libido!

[visionquest99]

So what precisely can this do for us? Anyone else used it/tried it?

[monty]

Got mine in the post today. Did my first shot this morning!

Not a sexual tyranasaurus yet!

[sanfran55]

I Tried it about 2-3 months into this whole ordeal, I did feel better on it and it gave me solid night erections but nothing lasted.

[monty]

did you do shot or cream?

[visionquest99]

Never tried either but according to my research, the “topical” is far superior to the “liqua vade”.

[monty]

I heard the opposite. Liquid 70% bio availability compared to trans dermal 30%!!!???

[visionquest99]

@monty

I heard the opposite. Liquid 70% bio availability compared to trans dermal 30%!!!???

That’s what the sales material for this product states. Most of the user reviews are different. I just ordered the topical stuff. Hope to have it sometime next week. Will report back. Good luck to all.

[sanfran55]

@monty

did you do shot or cream?

Shot.

[visionquest99]

Just got Sustain Alpha topical. For those using this, where do you apply it?

I’ve read anywhere from your shoulders, upper back, chest to your balls.

[monty]

Apparantly inside of the biceps and in between toes gives most absorption

[visionquest99]

@monty

Apparantly inside of the biceps and in between toes gives most absorption

Thanks Monty. I’ll try that.

A day after my first dose of Sustain, I did enjoy an increase in libido and a better quality erection.

However, I did feel/notice that my pecs were bigger, almost like the start of gyno.

I think low doses of Sustain (in my case) would be optimal but for now I’ve stopped taking it, as I have to do some blood tests and I dont want the Sustain to skew my results. I’ll wait a week then go for the test.

[cando]

Any updates? Worth a shot?