Probiotic Dosage to Mimic Fecal Transplant

General Discussion
#21

Some more thoughts looking at the 10 billion or more per day dosage.
You can also see they can recover this strain from tissue biopsies, not just the feces.
They’re basically saying 1 billion per day at 8 weeks might not be enough for effect.

Fecal excretion of Bifidobacterium infantis 35624 and changes in fecal microbiota after eight weeks of oral supplementation with encapsulated probiotic

using B. infantis 35624 transformed with a rifampicin resistance gene as a marker for enumeration, the investigators demonstrated that the strain had established itself in the colon of these patients, reaching levels of 105–108 cfu, depending on the individual; the strain could be recovered from fecal samples and colon biopsies.

Consequently, our study does not allow firm conclusions about the impact of B. infantis 35624 supplementation on the GI tract microbiota. However, exploratory studies from our laboratory, which characterized fecal microbiota using terminal restriction fragment polymorphism (t-RFLP) analysis of microbial DNA, showed that controlled daily administration of 1010 cfu B. infantis 35624 in milk for three weeks helped normalize the intestinal microbiota. The analysis of fecal samples from 24 subjects (13 with IBS and 11 controls) revealed 3 DNA fragments associated exclusively with IBS, 29 fragments that differed in proportion between IBS patients and healthy controls prior to treatment and a probiotic-associated shift to equivalent proportions of 16 of these 29 fragments in the microbiota of both groups (unpublished data). Hence, more robust and comprehensive surveys (and, possibly, longer interventions) may be needed to detect potential changes in GI tract microbiota associated with B . infantis 35624 supplementation

We conclude that further fecal microbiota studies with more comprehensive microbial surveys and additional doses of B. infantis 35624 administered for longer durations are needed to further explore the role of the intestinal microbiota composition and metabolic processes

I think ive posted this one before,

Suppression of gut dysbiosis by Bifidobacterium longum alleviates cognitive decline in 5XFAD transgenic and aged mice

https://www.nature.com/articles/s41598-019-48342-7

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#22

best thing to do is take inulin (prebiotics), lots of fibre and cut out sugar. The bacteria is already there, it just needs the right environment. No need to spend hundreds of dollars on supplements

#23

The thought is missing or lost bacteria.
The goal would be to reestablish select bacteria.
Inulin could be non-selective as it could be a growth factor for numerous species both beneficial and not. If some have decreased intestinal motility, inulin would not be recommended.
Most prebiotics are not recommended in the case of SIBO for example.
One thought on inulin and some other prebiotics is it could act as a decoy or binder for some types of pathogenic bacteria to literally flush out or act as a clearance pathway.

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#24

Here’s another example of differing stool and mucosal microbiomes in a disease state, so to just look at the stool (or treat with fmt) might not be enough.

Colonic mucosal microbiome differs from stool microbiome in cirrhosis and hepatic encephalopathy and is linked to cognition and inflammation

#25

@guitarman01 just opening up this topic out of interest. What is your opinion on the topics discussed in this thread today? :slight_smile: Did you or anyone else try a treatment with the certain bacterias / pro-biotics discussed here? If so, what have been their results? :slight_smile:

#26

You can use the search function to look for people’s experiences with probiotics, I’m sure there are plenty of them and they have universally led to no improvements.

#27