Researched this…
LINK: sekisui.com.sg/product_pages … 3aHSD.html
3-Hydroxysteroid Dehydrogenase ( 3-HSD )
3-Hydroxysteroid : NAD§+ Oxidoreductase [EC1.1.1.50]
Origin
Peudomonas testosteroni
Preparation and Specification
Appearance : White amorphous pouder (Lyophilized powder)
Activity : More than 90 units/mg protein
Properties
Molecular weight : Approx. 37,000Da
Optimum pH : 10.2 ~ 10.5
Optimum temp. : 50°C
pH stability : 6.0 ~ 9.5 (30°C, 17hr)
Thermal stability : below 50°C (pH7.2, 10min)
Michaelis constant : Androsterone 6.7×10-6M
: Na-cholate 8.3×10-6M
: NAD 6.7×10-5M
Storage and Stability
Below at 5°C : 1 year
: For prolonged storage, keep at -20°C
At room temp. : At least 1 week
Contaminants
Malatedehydrogenase : below 0.01%
Alcoholdehydrogenase : below 0.01%
Lactatedehydrogenase : below 0.01%
ß-Hydroxysteroidedehydrogenase : below 0.5%
Unit definition
One unit is the amount of enzyme required to oxidize 1µ mol of androsteron as a substrate per minute in the presence of NAD at pH 8.9 at 25°C.
Package
Activity assay principle
100 units/bottle
1,000 units/bottle